abstract：BACKGROUND:Hepatitis C virus (HCV) is one of the most common viral infections worldwide causing major human chronic pathology. Viral RNA can be detected 1-3 weeks after infection and in cases where HCV RNA is still detectable after 6 months, the patient is considered to be chronically infected. Early detection is cruci...

journal_title：Clinical chemistry and laboratory medicine

authors： Bashiardes S,Richter J,Christodoulou CG

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Usually, laboratory diagnostics of hereditary hemoglobin disorders is fairly straightforward. Sometimes, however, correct diagnosis can be difficult. In this study, we describe a family with multiple mutations and sequence variations in the alpha- and beta-globin gene clusters. METHODS:Hemocytometry results...

journal_title：Clinical chemistry and laboratory medicine

authors： de Vooght KM,van Wijk R,Rijksen G,van Solinge WW

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Mid-regional pro-adrenomedullin (MR-proADM) and endothelin-1 have been shown to predict mortality of patients with acute myocardial infarction. However, the prognostic value of both biomarkers in predicting long-term clinical events after acute myocardial infarction remains unclear. METHODS:In a prospective...

journal_title：Clinical chemistry and laboratory medicine

authors： Behnes M,Papassotiriou J,Walter T,Fiedler E,Sauer T,Lang S,Elmas E,Hoffmann U,Borggrefe M,Brueckmann M

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Several drugs may affect the diagnosis of brain death by depressing the electroencephalographic signal. Serum levels of these drugs must be below their respective therapeutic ranges. METHODS:A high performance liquid chromatography-based fast and simple method was developed for determination of thiopentone,...

journal_title：Clinical chemistry and laboratory medicine

authors： Meinitzer A,Kalcher K,Gartner G,Halwachs-Baumann G,März W,Stettin M

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:The calibration of Jaffe method for serum creatinine using one serum-based standard complemented with artificial matrix compensating standard's Jaffe-interfering substances allows two-point calibration with results well comparable with enzymatic methods. METHOD:Spectrophotometry was used. RESULTS:Jaffe pro...

journal_title：Clinical chemistry and laboratory medicine

authors： Chromý V,Rozkosná K,Sedlák P

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Angiotensin II is produced primarily by angiotensin I-converting enzyme (ACE) within atherosclerotic lesions and ACE level in plaques correlates with the severity of vessel wall damage. Therefore, we investigated the possible association of ACE gene insertion/deletion (I/D) polymorphism and the severity of a...

journal_title：Clinical chemistry and laboratory medicine

authors： Niemiec P,Zak I,Wita K

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Transition from communications (abstracts) of an Argentinean congress and their publication as full papers was analyzed. No similar report was found for clinical laboratory in Latin America. METHODS:A total of 388 communications from five congresses were identified, and searches were performed on the intern...

journal_title：Clinical chemistry and laboratory medicine

authors： Martínez AM,Fink NE

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:There is a need for reference materials (RMs) in the field of genetic testing for verification of test results obtained in patients and probands. For the frequent genetic variation G20210A in the prothrombin gene, it has been shown that purified plasmids containing the gene fragment harbouring the mutation c...

journal_title：Clinical chemistry and laboratory medicine

authors： Gancberg D,Corbisier P,Meeus N,Marki-Zay J,Mannhalter C,Schimmel H

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Blood alcohol determination plays an important role in laboratory medicine and forensic medicine. Nowadays, many methods are being used for alcohol measurement, but these methods are time-consuming and complex to perform laborious sample pre-treatment. The disposable amperometric biosensor, due to its portab...

journal_title：Clinical chemistry and laboratory medicine

authors： Luo P,Xie G,Liu Y,Xu H,Deng S,Song F

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Electrospray ionization mass spectrometry (ESI-MS) can be used for the measurement of glycated adult hemoglobin. Here, we describe the evaluation of ESI-MS for measurement of glycated (GHbF) and acetylated (AcHbF) fetal hemoglobin and the identification by mass of different chains of fetal hemoglobin. METHO...

journal_title：Clinical chemistry and laboratory medicine

authors： Davison AS,Green BN,Roberts NB

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Urinary enzymes, including the brush border enzyme alanine aminopeptidase (AAP), are reliable biomarkers of nephrotoxicity and represent early indicators of tubular damage. The aim of the present study was to develop and validate an automated urinary AAP activity assay. METHODS AND RESULTS:For this purpose,...

journal_title：Clinical chemistry and laboratory medicine

authors： Holdt B,Peters E,Nagel HR,Steiner M

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Accurate outcome prediction in gastroesophageal malignancies is challenging. Acute-phase response proteins (APRPs) have been claimed to be independent prognosticators, although the basis for their association with prognosis remains unexplained. We hypothesized that, similarly to pancreatic and lung cancers, ...

journal_title：Clinical chemistry and laboratory medicine

authors： Krzystek-Korpacka M,Matusiewicz M,Diakowska D,Grabowski K,Blachut K,Kustrzeba-Wojcicka I,Terlecki G,Gamian A

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:The usefulness of serum antibodies to common food antigens (immunoglobulin G4; IgG4) assay in management of patients suffering from food intolerance was assessed. METHODS:A total of 22 asymptomatic healthy subjects and 68 patients with symptoms referred for suspected food intolerance were studied. Serum IgG...

journal_title：Clinical chemistry and laboratory medicine

authors： Bernardi D,Borghesan F,Faggian D,Bianchi FC,Favero E,Billeri L,Plebani M

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:The plasma total and low-density lipoprotein-cholesterol (LDL-C) levels that are used as diagnostic criteria for familial hypercholesterolaemia (FH) probands in the general population are too stringent for use in relatives, given the higher prior probability of a first-degree relative being FH (50% vs. 1/500...

journal_title：Clinical chemistry and laboratory medicine

authors： Starr B,Hadfield SG,Hutten BA,Lansberg PJ,Leren TP,Damgaard D,Neil HA,Humphries SE

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Established general risk score models for intensive care patients incorporate several clinical and laboratory data. However, the collection, documentation and classification of clinical data are time-consuming, incur labor-related costs, and are dependent on the experience of the examiner. Therefore, in the ...

journal_title：Clinical chemistry and laboratory medicine

authors： Stachon A,Segbers E,Hering S,Kempf R,Holland-Letz T,Krieg M

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Pleomorphic adenoma (PA) is a benign tumour of the salivary gland with a tendency to malignancy which creates many diagnostic problems. N-acetyl-beta-hexosaminidase (HEX) is a lysosomal exoglycosidase involved in degradation of oligosaccharide chains of glycoproteins, glycolipids and glycosaminoglycans, know...

journal_title：Clinical chemistry and laboratory medicine

authors： Borzym-Kluczyk M,Olszewska E,Radziejewska I,Lewszuk A,Zwierz K

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:The compatibility of immunoassay tests in different sample matrices is extremely important during the assay validation process. In this study, we investigated the interchangeability of some Access (and therefore all the UniCel Family platforms) assays between serum and plasma. METHODS:We tested approximatel...

journal_title：Clinical chemistry and laboratory medicine

authors： Vignati G,Chiecchio A,Osnaghi B,Giovanelli L,Meloncelli C

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Fatty acid metabolites play a key role in numerous physiological and pathological processes. A rapid liquid chromatography-mass spectrometry assay for the simultaneous determination of prostanoids, isoprostane and lipoxygenase (LOX) derived fatty acid metabolites in a small biological sample of only 20 micro...

journal_title：Clinical chemistry and laboratory medicine

authors： Unterwurzacher I,Koal T,Bonn GK,Weinberger KM,Ramsay SL

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Mutations in BRCA1 and BRCA2 genes are associated with family predisposition to breast and ovarian cancer. Novel screening methods are required for efficient and rapid detection of sequence variants in cancer patients and their family members. METHODS:The screening for variants in the breast and ovarian can...

journal_title：Clinical chemistry and laboratory medicine

authors： Cvok ML,Cretnik M,Musani V,Ozretic P,Levanat S

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Pneumatic tube transport of blood samples reduces turnaround times and labour. However, the preanalytical effects on new clinical chemistry parameters and instruments are not fully known. The aim of this study was to evaluate the effect of pneumatic tube transport on haematology and coagulation parameters, i...

journal_title：Clinical chemistry and laboratory medicine

authors： Wallin O,Söderberg J,Grankvist K,Jonsson PA,Hultdin J

更新日期：2008-01-01 00:00:00

abstract：:Authors are usually invited to follow the instructions to authors in scientific journals, because this section is likely to contain unique requirements, such as the references style requested. Authors not only must accurately check the instructions, but are also expected to verify references against the original docum...

journal_title：Clinical chemistry and laboratory medicine

authors： Salvagno GL,Lippi G,Montagnana M,Guidi GC

更新日期：2008-01-01 00:00:00

abstract：:Successful reduction of the risk of viral transmission via blood components has focused attention on the risk of transfusion-associated bacterial sepsis. The incidence of transfusion reactions due to bacteria is currently estimated at between 1 per 100,000 and 1 per 1,000,000 units in the case of packed red blood cell...

journal_title：Clinical chemistry and laboratory medicine

authors： Walther-Wenke G

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Adult-type hypolactasia is characterized by the inability to digest lactose during adulthood, due to lactase (LCT) deficiency. It is usually diagnosed by the measurement of breath hydrogen increase after a lactose load (breath hydrogen test, BHT). A substitution of C to T at position -13910 bp upstream the L...

journal_title：Clinical chemistry and laboratory medicine

authors： Mottes M,Belpinati F,Milani M,Saccomandi D,Petrelli E,Calacoci M,Chierici R,Pignatti PF,Borgna-Pignatti C

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Previous genomic linkage studies have produced evidence linking sodium-lithium countertransport activity (Na/Li CT) with various chromosomal regions including loci harbouring glutathione S transferase (GST) genes. The aim of this study was to examine the putative association of erythrocyte Na/Li CT activity ...

journal_title：Clinical chemistry and laboratory medicine

authors： Goulas A,Kosmidou M,Hatzitolios AI,Raikos N,Molyva D,Savopoulos C,Giannopoulos S,Fidani L

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Genetic predisposition to hemochromatosis involves several different point mutations in the HFE gene. Routine testing for two such mutations (H63D and S65C) using real-time genotyping deserves special care, as these mutations are in close proximity (6 bp) of each other. METHODS:A novel assay was designed fo...

journal_title：Clinical chemistry and laboratory medicine

authors： Klaassen CH,van Aarssen YA,van der Stappen JW

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Genes correlated with nicotine may be important in obesity because of the role of nicotine in food intake. METHODS:To determine if single nucleotide polymorphisms (SNPs) in the neuronal nicotinic acetylcholine receptor alpha2 subunit gene (CHRNA2) are associated with overweight/obesity, 156 non-obese and 20...

journal_title：Clinical chemistry and laboratory medicine

authors： Kim J

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Apolipoprotein E (APOE) and choline acetyltransferase (ChAT) have been suggested as candidate genes for determining the risk of late-onset Alzheimer's disease. The aim of this study was to simultaneously detect polymorphisms in codons 112 and 158 of APOE and codon 2 of ChAT by hybridization probe multiplexin...

journal_title：Clinical chemistry and laboratory medicine

authors： Park HD,Park KU,Kim KW,Song J,Chang HE,Heo SR,Lee HJ,Kim JQ

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The European Directive 98/79/EC on in-vitro diagnostic medical devices (IVDs) regulates IVD marketing practices and post-market surveillance. IVD manufacturers have to inform the responsible Competent Authorities of any issues. In Germany, the Federal Institute for Drugs and Medical Devices (BfArM) is respon...

journal_title：Clinical chemistry and laboratory medicine

authors： Siekmeier R,Lütz J

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:There is a certain difference in opinion regarding the optimal choice of housekeeping genes used as normalization factors in gene expression analysis. We have therefore examined the suitability of three housekeeping genes, hypoxanthine phosphoribosyl transferase, beta-glucuronidase and beta2-micro-globulin, ...

journal_title：Clinical chemistry and laboratory medicine

authors： Kågedal B,Farnebäck M,Håkansson A,Gustafsson B,Håkansson L

更新日期：2007-01-01 00:00:00

abstract：:The early diagnosis of rheumatoid arthritis (RA) has become a priority owing to the availability of effective disease-modifying agents that can improve patient wellbeing and influence the clinical outcome. However, this represents a real challenge, as no clinical, radiological or immunological features are pathognomon...

journal_title：Clinical chemistry and laboratory medicine

authors： Bizzaro N

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The presence of fibronectin fragments has been observed in some inflammatory diseases and is believed to reflect tissue breakdown. In this study, possible fibronectin fragmentation and alterations in its domain and sialyl and fucosyl glycotope expressions were analyzed in amniotic fluids in relation to intra...

journal_title：Clinical chemistry and laboratory medicine

authors： Hirnle L,Katnik-Prastowska I

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The aim of our study was to determine the frequency of anti-thyroid-stimulating hormone (TSH) receptor antibodies (TRAb) in Tunisian patients with Graves' disease (GD) and to compare the validity of TRAb to that of thyroperoxidase (TPO-Ab) and thyroglobulin antibodies (TG-Ab). METHODS:ELISA was used to dete...

journal_title：Clinical chemistry and laboratory medicine

authors： Mankaï A,Toumi D,Chadli-Chaieb M,Ghedira-Besbes L,Saad F,Ouertani M,Sfar H,Jeddi M,Chaieb L,Ghedira I

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The aim of the current study was to investigate levels of adenosine deaminase in plasma of patients with hydatidiform mole. METHODS:Plasma adenosine deaminase levels were determined in 17 women with normal pregnant course, in 17 women with hydatidiform mole, and in 17 non-pregnant healthy volunteers. RESUL...

journal_title：Clinical chemistry and laboratory medicine

authors： Engin-Ustün Y,Ustün Y,Karabulut AB,Doğan K,Meydanli MM,Sezgin B

更新日期：2007-01-01 00:00:00

abstract：:Haemophilia A is the most common inherited bleeding disorder caused by defects in the F8C gene that encodes coagulation factor VIII. This X-linked recessive disorder occurs in approximately 1:5000 males. Haemophilia A is diagnosed based on normal prothrombin time, altered activated partial thromboplastin time and redu...

journal_title：Clinical chemistry and laboratory medicine

authors： Castaldo G,D'Argenio V,Nardiello P,Zarrilli F,Sanna V,Rocino A,Coppola A,Di Minno G,Salvatore F

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Selective serotonin reuptake inhibitors (SSRIs) in general, and citalopram/escitalopram in particular, are widely used to treat clinical depression. However, SSRI bioavailability and non-compliance represent major issues, especially in the clinical trials setting. In this context, frequent drug-level measure...

journal_title：Clinical chemistry and laboratory medicine

authors： Serebruany V,Malinin A,Dragan V,Atar D,van Zyl L,Dragan A,Lexapro Compliance Task Force.

更新日期：2007-01-01 00:00:00

abstract：:An alcohol-associated change in the serum transferrin glycoform pattern, carbohydrate-deficient transferrin (CDT), is used as a biomarker of chronic moderate to heavy alcohol consumption. A current limitation in CDT analysis is the lack of standardization, which hampers clinical and analytical comparison between studi...

journal_title：Clinical chemistry and laboratory medicine

authors： Jeppsson JO,Arndt T,Schellenberg F,Wielders JP,Anton RF,Whitfield JB,Helander A,International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Standardization of Carbohydrate-deficient Transferin (IFCC-WG-CDT).

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Elevated plasma total homocysteine is a risk factor for cardiovascular disease (CVD), but the randomized trials of dietary supplementation with B-vitamins to lower homocysteine have not yet provided clear evidence of benefit on vascular risk. METHODS:Cumulative meta-analysis of all randomized trials assessi...

journal_title：Clinical chemistry and laboratory medicine

authors： Clarke R,Armitage J,Lewington S,Collins R,B-Vitamin Treatment Trialists' Collaboration.

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Various studies have described decreased serum angiotensin-converting enzyme (ACE) activity in patients with pneumonia. The aim of the present study was to evaluate the role of ACE in pneumonia by comparing ACE insertion/deletion (I/D) genotype corrected serum ACE activity and to establish whether the severi...

journal_title：Clinical chemistry and laboratory medicine

authors： van de Garde EM,Endeman H,Deneer VH,Biesma DH,Ruven HJ,van Velzen-Blad H,Leufkens HG,van den Bosch JM

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Metabolic syndrome is closely related to several disturbances in lipid and lipoprotein metabolism. The aim of this study was to determine the association between apolipoprotein E (apoE) genotypes and the risk of metabolic syndrome and/or coronary heart disease complications. METHODS:The study included 279 s...

journal_title：Clinical chemistry and laboratory medicine

authors： Sima A,Iordan A,Stancu C

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The arginine derivatives asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) interfere with endothelial nitric oxide synthesis. Plasma ADMA and SDMA have been shown to be risk factors for cardiovascular disease and/or kidney function deterioration in a variety of patient populations. ME...

journal_title：Clinical chemistry and laboratory medicine

authors： Wang S,Vicente FB,Miller A,Brooks ER,Price HE,Smith FA

更新日期：2007-01-01 00:00:00